Fig. 1

Propagation of liver, spleen, lung, and brain Mø in co-culture and subculture with their respective stromal cells in tissue culture dishes and their segregation using bacteriological Petri dishes. a: Liver cells after passage 2, 21 days after seeding in a tissue culture dish. b: Spleen cells after passage 8, 8 days after seeding in a tissue culture dish. c: Lung cells after passage 2, 8 days after seeding in a tissue culture dish. d: Primary brain cells 28 days after seeding (left panel) and brain cells after passage 1, 23 days after seeding (right panel) in a tissue culture dish. e–h: Liver, spleen, lung, and brain Mø in bacteriological Petri dishes. Mø selectively adhered to the dish surface and non-adherent cells formed cell aggregates. Cell aggregates were removed by washing with conditioned medium except for the left panel of F. e: Liver Mø segregated from liver stromal cells after passage 3 of subculture. f: Spleen Mø segregated from spleen stromal cells after passage 5 of subculture. g: Lung Mø segregated from lung stromal cells after passage 3 of subculture. h: Brain Mø segregated from brain stromal cells after passage 1 of subculture