Fig. 5
ILC2 transfers to apoE−/− mice reduce the lipid content of atherosclerotic lesions. Quantification of atherosclerotic lesions of hematoxylin/eosin stained subvalvular heart (a) and brachiocephalic artery (BCA) sections (d) of apoE−/− mice fed a high fat diet for 9 weeks. The mice received 4 i.p. ILC2 transfers (0.5 × 106 cells/transfer) or equal volume of PBS during that time period until euthanasia at 16–17 weeks of age. Neutral lipid content of subvalvular heart (b, c) and BCA (e, f) sections of apoE−/− mice that received ILC2s (or PBS as a control) performed with Oil Red O (ORO) staining and respective representative pictures of stainings (c, f). Siglec F (g) content in subvalvular heart sections of apoE−/− mice that received ILC2s (or PBS as a control) and representative immunohistochemistry pictures (h) for both groups. Scale bar 100 μm in all cases. Anti-PC IgM levels in the plasma of mice that received ILC2s or PBS as a control (i). Mann Whitney U test, *P < 0.05. Each data point represents one mouse