Fig. 4

Effect of AF on BCR/NF-κB pathway in lung tissue of cold stress-induced rats (n = 6 per group). a Protein expression levels of Lyn, p-Akt, t-Akt, TNF-α, NF-κB p65 nuclei and NF-κB p65 total by western blotting. b-e Quantification of relative changes in protein expression. f AF decreased the level of COX-2 in lung tissue of cold stress-treated rats. Sections are 5 μm thick and photomicrographs are taken at 200×. Representative COX-2 staining in pneumonocytes by immunofluorescence. COX-2-positive cells were labeled by red fluorescence, DAPI restrained cell nuclei were labeled with blue fluorescence. g The ratio of COX-2-positive cells in each group. The data are presented as the mean ± SD. ** P < 0.01, * P < 0.05 vs. cold group; ## P < 0.01, # P < 0.05 vs. control group