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Fig. 3 | BMC Immunology

Fig. 3

From: Prostaglandin E2 stimulates COX-2 expression via mitogen-activated protein kinase p38 but not ERK in human follicular dendritic cell-like cells

Fig. 3

p38 MAPK is necessary for the PGE2-induced COX-2 expression in FDC-like cells. a The effects of ERK and p38 knockdown on COX-2 induction by PGE2 were examined with FDC-like cells that had been transfected with control or siRNA against ERK or p38 before the addition of PGE2 (1 μM). b The effects of PD098059 (PD, 50 μM) and SB203580 (SB, 10 μM) on COX-2 induction by PGE2 were examined. FDC-like cells (1 × 105 cells) were cultured in the presence of PD098059 or SB203580 for 30 min and then added with PGE2 (1 μM) for 8 h. The expression levels of COX-1, COX-2, and β-actin were measured by immunoblotting. Representative immunoblots and statistical analysis data (mean ± SEM) of three independent experiments are shown. An asterisk(s) indicates a significant difference (*, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, non-significant). v, vehicle

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BMC Immunology

ISSN: 1471-2172

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