Fig. 3

Recombinant human interleukin (IL)-35 stimulation to proliferation, cytokine/granzyme production, and death ligands mRNA expression in CD14⁺ monocytes from patients with Kawasaki disease (n = 11). CD14⁺ monocytes from peripheral bloods of patients with Kawasaki disease were stimulated with recombinant human IL-35 (50 ng/ml) and 1 × lipopolysaccharide for 24 h. a Cellular proliferation was measured by Cell Counting Kit-8 (CCK-8). CD14⁺ monocytes proliferation, which presented by OD_450nm, was comparable between cells with and without IL-35 stimulation. b Tumor necrosis factor-α (TNF-α) and granzyme production, including c granzyme A, d granzyme B, e granzyme H, and f granzyme K in cultured supernatants was measured by enzyme linked immunosorbent assay. b TNF-α and (c) granzyme B concentration in cultured supernatants was decreased in response to IL-35 stimulation. c Granzyme A, e granzyme H, and f granzyme K concentration was comparable between CD14⁺ monocytes with and without IL-35 stimulation. mRNA corresponding to g Fas ligand (FasL) and h TNF-related apoptosis-inducing ligand (TRAIL) was semi-quantified by real-time reverse transcriptional polymerase chain reaction. There were no significant differences of g FasL or h TRAIL mRNA relative level between CD14⁺ monocytes with and without IL-35 stimulation. Paired t test was used for comparison. Individual level of each subject was shown. The horizon line presented mean, and error bar presented standard deviation