Fig. 4

The expression levels of rejection and tolerance-related cytokine genes in allografts were detected using qRT-PCR and the levels of rejection and tolerance-related cytokines in the sera of the recipients were detected using ELISA. On day 4 post-transplantation, allografts and sera were harvested from the skin-primed recipients. a Expression levels of the genes encoding IL-2, IFN-γ, IL-4, IL-10, TGF, TNF-α, and Foxp3 within the allografts. b Expression levels of the genes encoding Fasl, Granzyme B, and Perforin within the allografts. c The protein levels of IL-4, IL-2, IFN-γ, TGF-β, and IL-10 in the recipient sera. The reactions were repeated three times and the data represent three separate experiments (n = 3 mice per group; *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus skin-primed HTm ctrl)