Table 2 qRT-PCR primers used in the present study
Sequences of the primers (5′–3′) | ||
|---|---|---|
Target gene | Forward | Reverse |
β-actin | CATCCGTAAAGACCTCTATGCCAAC | ATGGAGCCACCGATCCACA |
TNF-α | CATCTTCTCAAAATTCGAGTGACAA | TGGGAGTAGACAAGGTACAACCC |
IFN-γ | CGGCACAGTCATTGAAAGCCTA | GTTGCTGATGGCCTGATTGTC |
IL-2 | GGAGCAGCTGTTGATGGACCTAC | AATCCAGAACATGCCGCAGAG |
IL-4 | TCTCGAATGTACCAGGAGCCATATC | AGCACCTTGGAAGCCCTACAGA |
IL-10 | GACCAGCTGGACAACATACTGCTAA | GATAAGGCTTGGCAACCCAAGTAA |
FOXP3 | CAGCTCTGCTGGCGAAAGTG | TCGTCTGAAGGCAGAGTCAGGA |
TGF-β | TGACGTCACTGGAGTTGTACGG | GGTTCATGTCATGGATGGTGC |
FasL | GCCCATGAATTACCCATGTCC | ACAGATTTGTGTTGTGGTCCTT |
Perforin | AACTCCCTAATGAGAGACGCC | CCACACGCCAGTCGTTATTGA |
Granzyme B | CCACTCTCGACCCTACATGG | GGCCCCCAAAGTGACATTTATT |