Fig. 4
From: Progranulin inhibits LPS-induced macrophage M1 polarization via NF-кB and MAPK pathways
Effect of TNF-α on M1 polarization and of anti-TNF-α on LPS-induced M1 polarization in RAW264.7 cells. Cells were treated with LPS or plus anti-TNF-α or simple rTNF-α at concentration of 20, 30, 40 ng/ml for 24 h and normal medium containing equal amount of PBS to rTNF-α and LPS groups was used as negative control. a, b CD86 expression was detected by flow cytometry. c, d The gene level of TNF-α (c) and iNOS (d) were determined with q-PCR. e-g The protein level of TNF-α (e, f) and iNOS (e, g) were revealed by Western blot. N = 3, P < 0.05 (*), P < 0.01 (**) or p < 0.0001(****)