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Fig. 5 | BMC Immunology

Fig. 5

From: Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection

Fig. 5

The impact of ACAT1 on the BCG-induced autophagy in RAW264.7 cells. The ACAT1-overexpressed and silenced RAW264.7 cells were infected with BCG at a dose of 10 for 12 h before the pathogen-induced autophagy was determined by accessing autophagy-related proteins. a Representative blots showed an inhibition of BCG-induced autophagy-related proteins ATG7, ATG5, LC3II/I and Beclin1 in RAW264.7 cells overexpressing ACAT1 (top panel), and the relative expression semi-quantified by a densitometry analysis (bottom panel) demonstrated an significant reduction of autophagy-related proteins in comparison with control cells. b Representative blots displayed an increase of BCG-induced autophagy-related proteins ATG7, ATG5, LC3II/I and Beclin1 in RAW264.7 cells with a silence of ACAT1 (top panel), and the relative expression semi-quantified by a densitometry analysis (bottom panel) demonstrated an significant increase of autophagy-related proteins in comparison with uninfected control cells. Data were expressed as mean ± SEM from three independent experiments. Compared to non-BCG-infected cells, *: p < 0.05; **: p < 0.01; compared to non-control lentivirus-infected cells, ∆: p < 0.05; ∆∆: p < 0.01

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BMC Immunology

ISSN: 1471-2172

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