Table 2 Antibody specificity (target), metal tag and panel information sorted by metal tag. *denotes antibodies that were self-conjugated, ** seven stimulated samples did not contain this antibody, therefore, CCR7 was excluded from unsupervised analysis but used to determine T cell subpopulations in remaining 21 samples
| Â | Isotope | Target |
|---|---|---|
Panel 1 (32 surface antibodies) | ||
 surface | 089Y | CD45 |
 surface | 141Pr | CD196 (CCR6) |
 surface | 142Nd | CD19 |
 surface | 143Nd | CD127 (IL-7Ra) |
 surface | 144Nd | CD69 |
 surface | 145Nd | CD4 |
 surface | 146Nd | IgD |
 surface | 147Sm | CD11c |
 surface | 148Nd | CD16 |
 surface | 149Sm | CD194 (CCR4) |
 surface | 151Eu | CD123 (IL-3R) |
 surface | 152Sm | ydTcell receptor |
 surface | 153Eu | CD185 (CXCR5) |
 surface | 154Sm | CD3 |
 surface | 155Gd | CD45RA |
 surface | 156Gd | CD183 (CXCR3) |
 surface | 158Gd | CD33 |
 surface | 159 Tb | CD161 |
 surface | 160Gd | CD28 |
 surface | 162Dy | CD27 |
 surface | 163Dy | CD294 (CRTH2) |
 surface | 164Dy | CD23 |
 surface | 167Er | CD197 (CCR7) |
 surface | 168Er | CD8a |
 surface | 169Tm | CD25 (IL-2R) |
 surface | 170Er | HLA-DR |
 surface | 172Yb | CD38 |
 surface | 173Yb | CD371 (CLEC12A) |
 surface | 174Yb | *IgG |
 surface | 175Lu | CD14 |
 surface | 176Yb | CD56 (NCAM) |
 surface | 209Bi | CD11b (Mac-1) |
 intracellular | 191Ir | Cell-ID intercalator |
 intracellular | 193Ir | Cell-ID intercalator |
 Live/dead | 194Pt | Cisplatin – live/dead |
Panel 2 (18 surface and 11 intracellular antibodies) | ||
 surface | 089Y | CD45 |
 surface | 142Nd | CD19 |
 surface | 143Nd | CD127 (IL-7Ra) |
 surface | 144Nd | CD69 |
 surface | 145Nd | CD4 |
 surface | 147Sm | CD11c |
 surface | 148Nd | CD16 |
 intracellular | 150Nd | IL-22 |
 intracellular | 151Eu | IL-5 |
 intracellular | 152Sm | TNFa |
 surface | 153Eu | CD185 (CXCR5) |
 surface | 154Sm | CD3 |
 surface | 155Gd | CD45RA |
 intracellular | 156Gd | *IL-10 |
 intracellular | 158Gd | IL-2 |
 intracellular | 159 Tb | *IL-13 |
 intracellular | 161Dy | IL-17A |
 intracellular | 162Dy | Foxp3 |
 intracellular | 163Dy | IL-4 |
 surface | 164Dy | CD23 |
 intracellular | 165Ho | IFNg |
 surface | 167Er | **CD197 (CCR7) |
 surface | 168Er | CD8a |
 surface | 169Tm | CD25 (IL-2R) |
 surface | 170Er | HLA-DR |
 intracellular | 172Yb | Ki-67 |
 surface | 174Yb | CD89 |
 surface | 175Lu | CD14 |
 surface | 176Yb | CD56 (NCAM) |
 intracellular | 191Ir | Cell-ID intercalator |
 intracellular | 193Ir | Cell-ID intercalator |
 Live/dead | 194Pt | Cisplatin – live/dead |