Fig. 1
HIF-1 α stabilization correlates with hypoxia induced chemokine receptor hypo-responsiveness of primary human and mouse B cells. Isolated primary human peripheral blood B cells (a) or splenic mouse B cells (b) were incubated for 24 hours at the indicated O2 levels and then subjected to chemotaxis assay. Shown is representative experiment from 3 independent experiments for each B cell source. Lysates from either human (c) or mouse (d) B cells were prepared and westerns performed to measure HIF-1 α levels with actin being used as a loading control. Shown is a representative blot