Fig. 2
HIF-1 α stabilization correlates with hypoxia induced chemokine receptor hypo-responsiveness of the RAMOS B cell line. RAMOS B cells were incubated at the indicated O2 levels for 24 hours and then subjected to (a) chemotaxis assay or (b) lysates were prepared and westerns performed to measure HIF-1 α levels with actin being used as a loading control. Shown is a representative blot. (c) Quantitation of relative HIF-1 α levels from three separate experiments. ***P <0.001; **P <0.01