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Table 2 Primers and probes sequences (5′-3′) used in this study

From: Ex vivo mRNA expression of toll-like receptors during latent tuberculosis infection

Primers and probes Sequence (5′-3′)
HuPO Primer (5′-3′) forward: GCT TCC TGG AGG GTG TCC
reverse: GGA CTC GTT TGT ACC CGT TG
Probe (5′-6-FAM---TAMRA-3′): TGC CAG TGT CTG TCT GCA GAT TGG
TLR-1 Primer (5′-3′) forward: CAG TGT CTG GTA CAC GCA TGG
reverse: TTT CAA AAA CCG TGT CTG TTA AGA G
Probe (5′-6-FAM---TAMRA-3′): TGC CCA TCC AAA ATT AGC CCG TTC C
TLR-2 Primer (5′-3′) forward: GGC CAG CAA ATT ACC TGT GTG
reverse: AGG CGG ACA TCC TGA ACC T
Probe (5′-6-FAM---TAMRA-3′): TCC ATC CCA TGT GCG TGG CC
TLR-4 Primer (5′-3′) forward: GAG CCT TTT CTG GAC TAT CAA G
reverse: TCC AAT GGG GAA GTT CTC TAG
Probe (5′-6-FAM---TAMRA-3′): AGA TTT GTC TCC ACA GCC ACC AGC
TLR-6 Primer (5′-3′) forward: GAA GAA GAA CAA CCC TTT AGG ATA GC
reverse: AGG CAA ACA AAA TGG AAG CTT
Probe (5′-6-FAM---TAMRA-3′): TGC AAC ATC ATG ACC AAA GAC AAA GAA CCT
TLR-9 Primer (5′-3′) forward: GGA CC + T CTG GTA CTG CTT CCA
reverse: AAG CTC GTT GTA CAC CCA GTC T
Probe (5′-6-FAM---TAMRA-3′) ACG ATG CCT TCG TGG TCT TCG ACA AA
  1. HuPO human acidic ribosomal protein, TLR toll-like receptor, FAM 6-carboxyfluorescein, TAMRA tetramethylrhodamine