Table 2 Primers and probes sequences (5′-3′) used in this study

HuPO Primer (5′-3′)

forward: GCT TCC TGG AGG GTG TCC

reverse: GGA CTC GTT TGT ACC CGT TG

Probe (5′-6-FAM---TAMRA-3′):

TGC CAG TGT CTG TCT GCA GAT TGG

TLR-1 Primer (5′-3′)

forward: CAG TGT CTG GTA CAC GCA TGG

reverse: TTT CAA AAA CCG TGT CTG TTA AGA G

Probe (5′-6-FAM---TAMRA-3′):

TGC CCA TCC AAA ATT AGC CCG TTC C

TLR-2 Primer (5′-3′)

forward: GGC CAG CAA ATT ACC TGT GTG

reverse: AGG CGG ACA TCC TGA ACC T

Probe (5′-6-FAM---TAMRA-3′):

TCC ATC CCA TGT GCG TGG CC

TLR-4 Primer (5′-3′)

forward: GAG CCT TTT CTG GAC TAT CAA G

reverse: TCC AAT GGG GAA GTT CTC TAG

Probe (5′-6-FAM---TAMRA-3′):

AGA TTT GTC TCC ACA GCC ACC AGC

TLR-6 Primer (5′-3′)

forward: GAA GAA GAA CAA CCC TTT AGG ATA GC

reverse: AGG CAA ACA AAA TGG AAG CTT

Probe (5′-6-FAM---TAMRA-3′):

TGC AAC ATC ATG ACC AAA GAC AAA GAA CCT

TLR-9 Primer (5′-3′)

forward: GGA CC + T CTG GTA CTG CTT CCA

reverse: AAG CTC GTT GTA CAC CCA GTC T

Probe (5′-6-FAM---TAMRA-3′)

ACG ATG CCT TCG TGG TCT TCG ACA AA