Fig. 5

The impact of NET on the activation of granulocytes. After the designated treatments (with or without healthy and SLE NET), the upregulation of CD11b expression on the cell surface of LPS-challenged neutrophils was compared. Although the differences between the untreated and either the patient or healthy NET-treated neutrophils were significant, the difference between CD11b expression in untreated and NCM-treated granulocytes was not significant. NET pretreatment alone (without LPS stimulation) did not change the CD11b expression on the neutrophils. NCM: NET control medium; refers to the collected medium from the unstimulated healthy and SLE neutrophils in the NET-inducing experiments. MFI: Mean Fluorescence Intensity