Fig. 6
From: CD100 modulates cytotoxicity of CD8+ T cells in patients with acute myocardial infarction
Influence of recombinant human matrix metalloproteinase-2 (MMP-2) stimulation to CD8+ T cells cytotoxicity from HLA-A02 restricted acute myocardial infarction (AMI) patients (n = 9). 104 of CD8+ T cells were stimulated with recombinant human MMP-2 in the presence or absence of anti-CD100 neutralization antibody, and were co-cultured with 5 × 104 HUVEC in a direct contact manner. LDH level in the cultured supernatants were measured using LDH Cytotoxicity Assay Kit. The percentage of HUVECs death was calculated, and was compared among groups. Individual level for each subject was shown. The horizon lines indicated means, while the error bars indicated standard deviations. One-way ANOVA and SNK-q test was used for statistical analysis