Table 1 Treatment strategies for RAW cells in TLR4 inhibition experiments
From: Lipopolysaccharide- TLR-4 Axis regulates Osteoclastogenesis independent of RANKL/RANK signaling
Treatment Group | Treatment at 0 h | Treatment at 24 h | Treatment at 48 h |
|---|---|---|---|
RANKL | RANKL (60 ng/mL) | RANKL (60 ng/mL) | RANKL (60 ng/mL) |
RANKL + TAK-242 | RANKL (60 ng/mL) | RANKL (60 ng/mL) + TAK-242 (5 μM/mL) | RANKL (60 ng/mL) + TAK-242 (5 μM/mL) |
LPS | RANKL (60 ng/mL) | LPS (5 μg/mL) | LPS (5 μg/mL) |
LPS + TAK-242 | RANKL (60 ng/mL) | LPS (5 μg/mL) + TAK-242 (5 μM/mL) | LPS (5 μg/mL) + TAK-242 (5 μM/mL) |