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Table 1 Treatment strategies for RAW cells in TLR4 inhibition experiments

From: Lipopolysaccharide- TLR-4 Axis regulates Osteoclastogenesis independent of RANKL/RANK signaling

Treatment Group Treatment at 0 h Treatment at 24 h Treatment at 48 h
RANKL RANKL (60 ng/mL) RANKL (60 ng/mL) RANKL (60 ng/mL)
RANKL + TAK-242 RANKL (60 ng/mL) RANKL (60 ng/mL) + TAK-242 (5 μM/mL) RANKL (60 ng/mL) + TAK-242 (5 μM/mL)
LPS RANKL (60 ng/mL) LPS (5 μg/mL) LPS (5 μg/mL)
LPS + TAK-242 RANKL (60 ng/mL) LPS (5 μg/mL) + TAK-242 (5 μM/mL) LPS (5 μg/mL) + TAK-242 (5 μM/mL)