Fig. 5
Protective efficacy of r0190 against B. anthracis H9401 spore challenge. Survival of GPs against 15 (a) or 30 (b) LD50 of B. anthracis H9401 challenge. GPs were immunized with r0190 (5 μg), rPA (5 μg) or a mixture of r0190 and rPA (5 μg each) by i.m. injection on days 0 and 28. Alum was used as a control. The protective efficacy was evaluated by challenging with 15 or 30 LD50 of B. anthracis H9401 by i.m. injection on day 28 after the final vaccination. Animals that survived for 14 days were considered survivors. ** P < 0.01 as compared with the control group. c, d Antigen-specific IgG antibody titers against PA or GBAA0190 were determined by ELISA. Groups were compared by multiple-comparison test after one-way ANOVA. *** P < 0.001 as compared with the alum-immunized group. ND, not detected. NS, not significant