Fig. 3

The influence of Tim-3 blockade on CD107a expression and IFN-γ production in CD4⁺ T and NK cells. a–b Relative mRNA expression of IFN-γ in NK and CD4⁺ T cells with Tim-3 blockade (n = 4). c Representative flow cytometry plots of expression of CD107a expression and IFN-γ production in CD4⁺ T and NK cells incubated with anti-human Tim-3 blocking antibody or IgG control. d–e IFN-γ production in CD4⁺ T and NK cells with Tim-3 blockade (n = 4). f–g CD107a expression on CD4⁺ T and NK cells with Tim-3 blockade (n = 4). All data analyzed using FacsDiva™ 8.0.3 (URL: www.bdbiosciences.com) and FlowJo™ 10.5.0 (URL: www.flowjo.com/flowjo-eula/). A Wilcoxon matched-pairs signed rank test (GraphPad Prism 9.0, URL: www.graphpad.com) was used for comparisons between two groups; ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001