Fig. 3

Effects of TREM2 on the differentiation of NK cells from HSCs in vitro. a Representative flow cytometry plots of NK cell receptor expression on the differentiated NK cells from WT-HSCs or TREM2-TG-HSCs. Cells treated with hu-Ig or TREM2-Ig during the pNK to mNK cell differentiation simultaneously. b Graphs indicate the total number of cells (upper panel) and percentage of NK1.1⁺ cells (lower panel) differentiated from WT or TG –HSCs, where were treated with hu-Ig (open bar) or TREM2-Ig (solid bar). c Graphs show the absolute cell number of the NK1.1⁺ cell population, NK receptor-expressing NK cells that were differentiated from WT-HSCs treated with hu-Ig (open bar) or TREM2-Ig (solid bar). d Real-time-qPCR analysis of IFN-γ, Perforin, Granzyme B, FasL, and TRAIL mRNAs in mNK cells derived from WT-HSCs or TG-HSCs. Values are presented as the mean ± standard error of the mean of three independent experiments (b-d). e Cytotoxic effect of differentiated NK cells derived from WT-HSCs and TREM2-HSCs treated with hu-Ig or TREM2-Ig since the pNK stage. *P < 0.05, **P < 0.01, ***P < 0.001 vs. WT-hu-Ig and †P < 0.05, ††P < 0.01, and †††P < 0.001 vs. TG-hu-Ig by two-way ANOVA analysis with Bonferroni posthoc test. Three independent experiments were performed