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Table 1 Flow cytometry profile of PBMCs (day 0) and T cells (day 28) of the three donors. The results are shown as mean ± SD

From: Optimizing interleukin-2 concentration, seeding density and bead-to-cell ratio of T-cell expansion for adoptive immunotherapy

 

CD3 (%)

CD4 (%)

CD8 (%)

CD3/CD4 (%)

CD3/CD8 (%)

CD4/CD8 (%)

PBMC

 Donor 1

57 ± 5.7

31.7 ± 2.4

26 ± 2.1

30.9 ± 6.4

21 ± 2

1.2 ± 0.5

 Donor 2

56.9 ± 2.4

30.2 ± 2.6

26.9 ± 1.6

27.1 ± 6.1

24.5 ± 1.8

1.7 ± 0.7

 Donor 3

56.8 ± 8.5

28.5 ± 4.3

27.4 ± 2.5

26.8 ± 12.8

25.6 ± 2.2

1.1 ± 0.2

T cell

 Donor 1

97.5 ± 1.8

19.3 ± 10.5

72.1 ± 19.4

17.3 ± 7.3

70.7 ± 13.4

1.34 ± 0.4

 Donor 2

96.6 ± 1.9

16.5 ± 9.9

70.4 ± 15.8

14.7 ± 8.7

65.8 ± 11.5

2.1 ± 0.3

 Donor 3

98 ± 1.3

20.4 ± 11.5

69.9 ± 17.7

16.7 ± 9.1

68.7 ± 10.3

1.5 ± 0.5