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Table 1 Flow cytometry profile of PBMCs (day 0) and T cells (day 28) of the three donors. The results are shown as mean ± SD

From: Optimizing interleukin-2 concentration, seeding density and bead-to-cell ratio of T-cell expansion for adoptive immunotherapy

  CD3 (%) CD4 (%) CD8 (%) CD3/CD4 (%) CD3/CD8 (%) CD4/CD8 (%)
PBMC
 Donor 1 57 ± 5.7 31.7 ± 2.4 26 ± 2.1 30.9 ± 6.4 21 ± 2 1.2 ± 0.5
 Donor 2 56.9 ± 2.4 30.2 ± 2.6 26.9 ± 1.6 27.1 ± 6.1 24.5 ± 1.8 1.7 ± 0.7
 Donor 3 56.8 ± 8.5 28.5 ± 4.3 27.4 ± 2.5 26.8 ± 12.8 25.6 ± 2.2 1.1 ± 0.2
T cell
 Donor 1 97.5 ± 1.8 19.3 ± 10.5 72.1 ± 19.4 17.3 ± 7.3 70.7 ± 13.4 1.34 ± 0.4
 Donor 2 96.6 ± 1.9 16.5 ± 9.9 70.4 ± 15.8 14.7 ± 8.7 65.8 ± 11.5 2.1 ± 0.3
 Donor 3 98 ± 1.3 20.4 ± 11.5 69.9 ± 17.7 16.7 ± 9.1 68.7 ± 10.3 1.5 ± 0.5