Fig. 2
Angiopoietin 1 release from neutrophils. Isolated neutrophils (5 × 106/mL) from HC, T2DM, stable HFpEF ± T2DM and ADHFpEF ± T2DM patients were incubated at 37 °C for 3 h with different agonists (PBS control, PMA, LPS and A23187). By ELISA, we quantified Ang1 from unstimulated neutrophils (T0) and stimulated neutrophils. §p < 0.05 and §§p < 0.01 versus T0