Fig. 3
Calprotectin synthesis and release from neutrophils. Isolated neutrophils (5 × 106/ml) from HC, T2DM, stable HFpEF ± T2DM and ADHFpEF ± T2DM patients were incubated at 37 °C for 3 h with different agonists (PBS control, PMA, LPS and A23187). By ELISA, we quantified calprotectin from unstimulated (T0) and stimulated neutrophils. Total calprotectin corresponds to the addition of calprotectin released, intracellular and bound to NETs. §p < 0.05, §§p < 0.01 and §§§p < 0.001 versus T0, *p < 0.05, **p < 0.01 and ***p < 0.001 versus PBS, †p < 0.05 versus HC-corresponding agonist, £p < 0.05 versus T2DM-corresponding agonist and ¥¥p < 0.01 versus HFpEF + T2DM