Table 4 Percentage positive antibody responses of the four representative DENV peptides of broadly immunogenic E protein epitopes to human sera of four DENV serotypes
Serotype of the sera | P4/E (72–88) | P5/E (89–104) | P14/E (394–410) | P15/E (418–434) | P17/E (458–474) |
|---|---|---|---|---|---|
DENV1 SRCPTQGEATLVEEQDA | DENV1 NFVCRRTFVDRGWGNG | DENV1 KGSSIGKMFEATARGAR | DENV1 TWADFGSIGGVFTSVGK | DENV1 IGILLTWLGLNSRSTSL | |
DENV1 | 12 (100%) | 12 (100%) | 10 (83%) | 12 (100%) | 10 (83%) |
DENV2 | 12 (100%) | 12 (100%) | 10 (83%) | 12 (100%) | 9 (75%) |
DENV3 | 12 (100%) | 12 (100%) | 12 (100%) | 12 (100%) | 9 (75%) |
DENV4 | 12 (100%) | 12 (100%) | 12 (100%) | 12 (100%) | 9 (75%) |
DENV2 RCPTQGEPSLNEEQDKRF | DENV2 KRFVCKHSMVDRGWGNGCGL | DENV2 KKGSSIGQMIETTMRGAK | DENV2 AILGDTAWDFGSLGGVF | DENV2 IITWIGMNSRSTSLSVSL | |
DENV1 | 12 (100%) | 12 (100%) | 10 (83%) | 0 (0%) | 12 (100%) |
DENV2 | 12 (100%) | 12 (100%) | 10 (83%) | 0 (0%) | 12 (100%) |
DENV3 | 12 (100%) | 12 (100%) | 10 (83%) | 0 (0%) | 12 (100%) |
DENV4 | 12 (100%) | 12 (100%) | 10 (83%) | 0 (0%) | 12 (100%) |
DENV3 SRCPTQGEAILPEEQDQNH | DENV3 CKHTYVDRGWGNGCGLF | DENV3 KKGSSIGKMFEATARGAR | DENV3 WDFGSVGGVLNSLGKMVH | DENV3 IGIGVLLTWIGLNSK | |
DENV1 | 12 (100%) | 0 (0%) | 12 (100%) | 6 (50%) | 0 (0%) |
DENV2 | 12 (100%) | 0 (0%) | 12 (100%) | 9 (75%) | 0 (0%) |
DENV3 | 12 (100%) | 0 (0%) | 12 (100%) | 10 (83%) | 0 (0%) |
DENV4 | 12 (100%) | 0 (0%) | 12 (100%) | 6 (50%) | 3(25%) |
DENV4 TRCPTQGEPYLKEEQDQQY | DENV4 CRRDVVDRGWGNGCGLF | DENV4 SIGKMFESTYRGAKRMAI | DENV4 WDFGSVGGLFTSLGKAVH | DENV4 LVLWIGTNSRNTSMAM | |
DENV1 | 2 (17%) | 9 (75%) | 0 (0%) | 10 (83%) | 0 (0%) |
DENV2 | 10 (83%) | 9 (75%) | 0 (0%) | 12 (100%) | 0 (0%) |
DENV3 | 2 (17%) | 10 (83%) | 0 (0%) | 9 (75%) | 1 (8%) |
DENV4 | 12 (100%) | 6 (50%) | 0 (0%) | 9 (75%) | 0 (0%) |
Serotype of the sera | P2/E (30–46) | P3/E (60–76) | P13/E (377–393) | P16/E (424–440) |
|---|---|---|---|---|
DENV1 CVTTMAKDKPTLDIELL | DENV1 CIEAKISNTTTDSRCPT | DENV1 YIVVGAGEKALKQCWFK | DENV1 SIGGVFTSVGKLVHQVF | |
DENV1 | 12 (100%) | 10 (83%) | 0 (0%) | 0 (0%) |
DENV2 | 9 (75%) | 0 (0%) | 12 (100%) | 12 (100%) |
DENV3 | 6 (50%) | 9 (100%) | 9 (75%) | 10 (83%) |
DENV4 | 0 (0%) | 12 (100%) | 9 (75%) | 10 (83%) |
DENV2 SCVTTMAKNKPTLDFELI | DENV2 EIKITPQSSITEAFLTGY | DENV2 FGDSYIIIGVEPGQLKL | DENV2 WDFGSLGGVFTSIGKALH | |
DENV1 | 3 (25%) | 10 (83%) | 1 (8%) | 0(0%) |
DENV2 | 12 (100%) | 12 (100%) | 6 (50%) | 0 (0%) |
DENV3 | 6 (50%) | 12 (100%) | 0 (0%) | 0 (0%) |
DENV4 | 0 (0%) | 9 (75%) | 6 (50%) | 0 (0%) |
DENV3 GCVTTMAKNKPTLDIEL | DENV3 LCIEGKITNVTTDSR7 | DENV3 KALKINWYKKGSSIGKMF | DENV3 VLNSLGKMVHQIFGSAY | |
DENV1 | 0 (0%) | 0 (0%) | 1 (8%) | 0 (0%) |
DENV2 | 0 (0%) | 0 (0%) | 5 (42%) | 2 (17%) |
DENV3 | 4 (33%) | 0 (0%) | 12 (100%) | 0 (0%) |
DENV4 | 5 (42%) | 0 (0%) | 0 (0%) | 3 (25%) |
DENV4 GCVTTMAQGKPTLDFEL | DENV4 SISNITTATRCPTQQGEPY | DENV4 FDSYIVIGVGNSALTLH | DENV4 WDFGSVGGLFTSLGKAVH | |
DENV1 | 2 (17%) | 9 (75%) | 0 (0%) | 0 (0%) |
DENV2 | 10 (83%) | 3 (25%) | 1 (8%) | 0 (0%) |
DENV3 | 3 (25%) | 10 (83%) | 0 (0%) | 0 (0%) |
DENV4 | 6 (50%) | 12 (100%) | 0 (0%) | 0 (0%) |