Table 4 Percentage positive antibody responses of the four representative DENV peptides of broadly immunogenic E protein epitopes to human sera of four DENV serotypes
| Serotype of the sera | P4/E (72–88) | P5/E (89–104) | P14/E (394–410) | P15/E (418–434) | P17/E (458–474) |
|---|---|---|---|---|---|
|
DENV1 SRCPTQGEATLVEEQDA |
DENV1 NFVCRRTFVDRGWGNG |
DENV1 KGSSIGKMFEATARGAR |
DENV1 TWADFGSIGGVFTSVGK |
DENV1 IGILLTWLGLNSRSTSL | |
| DENV1 | 12 (100%) | 12 (100%) | 10 (83%) | 12 (100%) | 10 (83%) |
| DENV2 | 12 (100%) | 12 (100%) | 10 (83%) | 12 (100%) | 9 (75%) |
| DENV3 | 12 (100%) | 12 (100%) | 12 (100%) | 12 (100%) | 9 (75%) |
| DENV4 | 12 (100%) | 12 (100%) | 12 (100%) | 12 (100%) | 9 (75%) |
|
DENV2 RCPTQGEPSLNEEQDKRF |
DENV2 KRFVCKHSMVDRGWGNGCGL |
DENV2 KKGSSIGQMIETTMRGAK |
DENV2 AILGDTAWDFGSLGGVF |
DENV2 IITWIGMNSRSTSLSVSL | |
| DENV1 | 12 (100%) | 12 (100%) | 10 (83%) | 0 (0%) | 12 (100%) |
| DENV2 | 12 (100%) | 12 (100%) | 10 (83%) | 0 (0%) | 12 (100%) |
| DENV3 | 12 (100%) | 12 (100%) | 10 (83%) | 0 (0%) | 12 (100%) |
| DENV4 | 12 (100%) | 12 (100%) | 10 (83%) | 0 (0%) | 12 (100%) |
|
DENV3 SRCPTQGEAILPEEQDQNH |
DENV3 CKHTYVDRGWGNGCGLF |
DENV3 KKGSSIGKMFEATARGAR |
DENV3 WDFGSVGGVLNSLGKMVH |
DENV3 IGIGVLLTWIGLNSK | |
| DENV1 | 12 (100%) | 0 (0%) | 12 (100%) | 6 (50%) | 0 (0%) |
| DENV2 | 12 (100%) | 0 (0%) | 12 (100%) | 9 (75%) | 0 (0%) |
| DENV3 | 12 (100%) | 0 (0%) | 12 (100%) | 10 (83%) | 0 (0%) |
| DENV4 | 12 (100%) | 0 (0%) | 12 (100%) | 6 (50%) | 3(25%) |
|
DENV4 TRCPTQGEPYLKEEQDQQY |
DENV4 CRRDVVDRGWGNGCGLF |
DENV4 SIGKMFESTYRGAKRMAI |
DENV4 WDFGSVGGLFTSLGKAVH |
DENV4 LVLWIGTNSRNTSMAM | |
| DENV1 | 2 (17%) | 9 (75%) | 0 (0%) | 10 (83%) | 0 (0%) |
| DENV2 | 10 (83%) | 9 (75%) | 0 (0%) | 12 (100%) | 0 (0%) |
| DENV3 | 2 (17%) | 10 (83%) | 0 (0%) | 9 (75%) | 1 (8%) |
| DENV4 | 12 (100%) | 6 (50%) | 0 (0%) | 9 (75%) | 0 (0%) |
| Serotype of the sera | P2/E (30–46) | P3/E (60–76) | P13/E (377–393) | P16/E (424–440) |
|---|---|---|---|---|
|
DENV1 CVTTMAKDKPTLDIELL |
DENV1 CIEAKISNTTTDSRCPT |
DENV1 YIVVGAGEKALKQCWFK |
DENV1 SIGGVFTSVGKLVHQVF | |
| DENV1 | 12 (100%) | 10 (83%) | 0 (0%) | 0 (0%) |
| DENV2 | 9 (75%) | 0 (0%) | 12 (100%) | 12 (100%) |
| DENV3 | 6 (50%) | 9 (100%) | 9 (75%) | 10 (83%) |
| DENV4 | 0 (0%) | 12 (100%) | 9 (75%) | 10 (83%) |
|
DENV2 SCVTTMAKNKPTLDFELI |
DENV2 EIKITPQSSITEAFLTGY |
DENV2 FGDSYIIIGVEPGQLKL |
DENV2 WDFGSLGGVFTSIGKALH | |
| DENV1 | 3 (25%) | 10 (83%) | 1 (8%) | 0(0%) |
| DENV2 | 12 (100%) | 12 (100%) | 6 (50%) | 0 (0%) |
| DENV3 | 6 (50%) | 12 (100%) | 0 (0%) | 0 (0%) |
| DENV4 | 0 (0%) | 9 (75%) | 6 (50%) | 0 (0%) |
|
DENV3 GCVTTMAKNKPTLDIEL |
DENV3 LCIEGKITNVTTDSR7 |
DENV3 KALKINWYKKGSSIGKMF |
DENV3 VLNSLGKMVHQIFGSAY | |
| DENV1 | 0 (0%) | 0 (0%) | 1 (8%) | 0 (0%) |
| DENV2 | 0 (0%) | 0 (0%) | 5 (42%) | 2 (17%) |
| DENV3 | 4 (33%) | 0 (0%) | 12 (100%) | 0 (0%) |
| DENV4 | 5 (42%) | 0 (0%) | 0 (0%) | 3 (25%) |
|
DENV4 GCVTTMAQGKPTLDFEL |
DENV4 SISNITTATRCPTQQGEPY |
DENV4 FDSYIVIGVGNSALTLH |
DENV4 WDFGSVGGLFTSLGKAVH | |
| DENV1 | 2 (17%) | 9 (75%) | 0 (0%) | 0 (0%) |
| DENV2 | 10 (83%) | 3 (25%) | 1 (8%) | 0 (0%) |
| DENV3 | 3 (25%) | 10 (83%) | 0 (0%) | 0 (0%) |
| DENV4 | 6 (50%) | 12 (100%) | 0 (0%) | 0 (0%) |