Fig. 3

Identification and functional clustering of the TFAM-regulated alternative splicing events. a Classification of the TFAM-regulated alternative splicing events (RASEs). b Plots of several representative RASEs regulated by TFAM. The schematic diagrams depict the structures of ASEs. Alternative splicing (AS) junctions, purple line; model splicing (Model) junctions, green line. The exon sequences are denoted by boxes and intron sequences by the horizontal line (top panel). RNA-seq quantification validation of ASEs are shown in the bottom panel. Error bars represent mean ± SEM. *p < 0.05, **p < 0.01. The altered ratio of AS events in RNA-seq were calculated using the formula: AS junction reads/(AS junction reads + Model junction reads). Plots of the top 10 GO biological processes (c) and KEGG (d) enriched by TFAM-regulated RASGs. e Venn diagram shows the overlap between the TFAM-regulated DEGs and TFAM-regulated RASGs; at least one TFAM-regulated RASE was present in each RASG. f The GO biological processes and KEGG pathways enriched by genes regulated by TFAM at both the transcriptional and alternative splicing levels