Fig. 6
Effect of immune sera on huIL-2-induced proliferation of CTLL-2 cells. CTLL-2 cells (5 × 103 cells/well) were cultured for 72 h with 50 ng/mL of huIL-2 and serial dilutions 1:2 (initial dilution 1:25) of the pool of sera collected 15 days after the third immunization from animals immunized with 200 µg of mhIL-15, 200 µg of IL-15 D8SQ108S or 350 µg of IL-15 D8SQ108S. Cell proliferation was measured by MTT staining. Data represent the mean values of OD578nm ± standard deviation (SD) from two independent experiments performed in triplicate. Dashed lines represent the minimum and maximum proliferation of the cells