Fig. 1

Effects of different anesthesia on the ratio of Th17/Treg cell in peripheral blood of lung cancer patients at different ages. A The flow scatter diagram of T6; B Th17/Treg ratio during perioperative period; C Levels of Th17-related cytokine (IL-17) and Treg-related cytokines (IL-10, TGF-β) during perioperative period were detected by ELISA. In brief, 5 mL venous blood was taken before anesthesia induction (T0), 10 min after anesthesia induction (T1), 1 h after the beginning of the operation (T2), 1 h after the end of operation (T3), the 1st day after operation (T4), the 3rd day after operation (T5) and the 7th day after operation (T6) respectively. Treg cells were screened from CD4⁺ T cells by CD25/Foxp3, with IgG acting as the isotype control. PBMCs cells were incubated with antibody CD3/CD8 and then added with fixed membrane penetrating agent. After washing and resuspending, PBMCs cells were added with antibody IL-17A to screen Th17 cells, with IgG acting as the isotype control. All experiments were repeated three times. Data in panels B/C were analyzed using one-way ANOVA, followed by Tukey's multiple comparisons test, ***p < 0.001