Fig. 2

Propofol affected the balance between Th17/Treg cells through GABAA receptor. A Results of trypan blue staining on CD4⁺ T cells showed that the dead cells was stained blue, while the living cells was not stained; B Expressions of RORγt and Foxp3 in CD4⁺ T cells were detected by qRT-PCR; C Ratio of Th17/Treg cell treated with propofol at different concentrations was detected using flow cytometry; the concentrations of P1, P2 and P3 were 0.3 μg/mL, 3 μg/mL and 30 μg/mL respectively; B1, B2 and B3 represented that 100 μM of bicuculline (GABAA receptor antagonist) was added on the basis of P1-P3. All experiments were repeated three times. Data in panel B/C were analyzed using one-way ANOVA, followed by Tukey's multiple comparisons test. Compared with the blank group, **p < 0.01, ***p < 0.001; compared with the inter groups, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001