Fig. 2

DHA feeding affected NK cell frequency, maturation and Ly49H⁺ NK cell expansion during the response to MCMV infection. A Flow cytometric analysis and enumeration of NK cells (CD45⁺CD3⁻CD19⁻ NK1.1⁺NKp46⁺) in the BM, spleens, pLNs and livers of control versus DHA-fed mice. B Flow cytometric analysis and cumulative frequencies of subpopulations of NK cell (CD3⁻CD19⁻NK1.1⁺NKp46⁺) subsets based on CD11b and CD27 expression in the BM, spleens, pLNs and livers of control versus DHA-fed mice. C Flow cytometric analysis of the KLRG1⁺ subsets of NK cells (CD3⁻CD19⁻NK1.1⁺NKp46⁺) in the BM, spleens, pLNs and livers of control versus DHA-fed mice at day 7 postinfection. D Flow cytometric analysis and enumeration of Ly49H⁺ subsets of NK cells (CD3⁻CD19⁻NK1.1⁺NKp46⁺) in the BM, spleens, pLNs and livers of control versus DHA-fed mice at day 7 postinfection. E Cumulative frequencies of NK cell (CD3⁻CD19⁻NK1.1⁺NKp46⁺) subsets found in the spleens, and livers of control versus DHA-fed mice at day 21 postinfection. For each experiment, n = 4 to 9 pooled from 2–3 independent experiments (A–E). Each symbol represents an individual mouse, and the blue dots and red square represent control and DHA-enriched diet-fed mice, respectively. Error bars represent interquartile ranges; *p < 0.05; **p < 0.01; ***p < 0.001