Fig. 1

STAT6 was activated in LPS-treated mice and macrophages. Wild type (WT) and STAT6 knock-out (KO) adult mice were intratracheal (i.t.) injected with 5 mg/kg LPS or PBS for 2 days. A Immunostaining for phosphorylated STAT6 (p-STAT6) in the lung tissues of treated mice. Representative photograph with 100 × magnification. B Quantitative analysis of p-STAT6 + cells in lung tissues after immunostaining. The total number of nuclei and those in p-STAT6 + cells were scored. At least 8 fields per sample were counted at 200 × magnification. Data was presentd as the percentage of nuclei in p-STAT6 + cells to total nuclei in one field. C Representative histogram of p-STAT6 + cells in the lung digests by flow cytometry. D Western blot analysis for the expression of total STAT6 in LPS-treated bone marrow-derived macrophages (BMDMs). GAPDH is internal loading control (upper panel). Quantitative analysis of toal STAT6 expression in BMDMs (lower panel). Data was presented as ratio of STAT6 densitometric density to GAPDH. E Immunostaining for p-STAT6 in BMDMs. Green: positively stained cells; Blue: DAPI-stained nuclei. Representative photograph with 200 × magnification. F Quantitative analysis of fluorescence intensity of p-STAT6 + cells by ImageJ software after immunostaining. Data was presented as ratio of fluorescence intensity to WT/0 group. All quantitative data was presented as mean ± standard error, n = 3–7, *p < 0.05 versus the untreated controls; #p < 0.05, ##p < 0.01 versus WT group. One-way ANOVA test followed by Tukey’s multiple comparisons