Fig. 2

Lack of STAT6 expression in KO mice induced more severe ALI. A Lung histology of WT and KO mice with LPS-induced ALI by H&E staining. Representative photograph with 100× magnification. Black arrows indicated the inflammatory infiltrates. B Quantitative analysis of lung inflammation in each group of mice. Lung injury severity was evaluated by scale from 0 to 4 in terms of infiltrating inflammatory cells and alveoli destruction. C Total cell counts in BAL. D Total protein content in BAL by BCA assay. E–G ELISA analysis for the expression of TNF-alpha, IL-6 and IL-1beta in BAL. All quantitative data was presented as mean ± standard error, *p < 0.05, **p < 0.01 versus PBS group, #p < 0.05 versus WT/LPS group, n = 3–7. One-way ANOVA test followed by Tukey’s multiple comparisons