Fig. 4

Lack of STAT6 expression in KO mice induced polarization of Siglec-F(−) subtype macrophages. A Flow cytometry analysis for the percentage of F4/80(low)Ly6G(high) neutrophils (NPs) and F4/80(high)Ly6G(low) macrophages (MPs) in BAL of murine ALI (upper panel). Siglec-F(−) and Siglec-F + subtype macrophages were gated on MPs (lower panel). Representative dot plot in each group. B Quantitative analysis for the percentage of NPs and MPs in the lung tissues of murine ALI after flow cytometry analysis. C Quantitative analysis for the percentage of Siglec-F(−) and Siglec-F + subtype macrophages in the lung tissues of murine ALI. All quantitative data was presented individual data ponts, *p < 0.05, **p < 0.01 versus PBS group; #p < 0.05, ##p < 0.01 versus WT group. One-way ANOVA test followed by Tukey’s multiple comparisons