Fig. 5

Lack of STAT6 increased the expression of NLRP3 and pro-inflammatory cytokines in bone marrow-derived macrophages (BMDMs). BMDMs from WT and STAT6 KO mice were treated with or without 500 ng/ml LPS for 24 h. A Western blot analysis for the expression of NLRP3 and p62 in the treated WT and KO BMDMs. B Quantitative analysis for the expression of NLRP3 and p62 in Western blots by ImageJ software. C Western blot analysis for p-p38 MAPK and total p38 MAPK in the treated WT and KO BMDMs. D Quantitative analysis for p-p38 MAPK and ratio of p-p38 MAPK/total p38 MAPK in Western blots by ImageJ software. Data was presented as ratio of densitometric density to GAPDH internal loading control. E ELISA analysis for the expression of TNF-alpha, IL-1beta and Calreticulin in the supernatants of treated cells. All quantitative data was presented as mean ± standard error. *p < 0.05 versus untreated group; #p < 0.05, ##p < 0.01 versus WT group, n = 3–4. One-way ANOVA test followed by Tukey’s multiple comparisons