Fig. 6

Effects of long-term cryopreservation on functional T cells-Th1, Th2, Th17(n = 12)and Tfh, Tregs(n = 18).The proportions of IFN-γ⁺Th1,IL-4⁺Th2,IL-17⁺Th17 (A) and CXCR5⁺Tfh (B) in fresh isolated and cryopreserved (1, 3 and 6 mo, respectively) PBMCs. C The proportions of CD4⁺CD25⁺Tregs expressing CD127⁻ or Foxp3⁺. D The frequency of Tregs, actived Tregs, proliferated Tregs after long-term cryopreservation. E Suppressive experiment using the CFSE label PBMCs cocultured with cryopreserved Tregs. F CD45RO⁻Naïve Tregs,CD45RO⁺Tregs in total CD4⁺T cells or in Tregs in fresh isolated and cryopreserved (1, 3 and 6 mo, respectively) PBMCs. *P < 0.05, **P < 0.01,***P < 0.001