Comprehensive evaluation of the effects of long-term cryopreservation on peripheral blood mononuclear cells using flow cytometry

Table 3 Effect of long-term cryopreservation on functional T cells

	Fresh (%)	1 m (%)		3 m (%)			6 m (%)
	Mean (range)	Mean (range)	Fresh versus 1 m P	Mean (range)	Fresh versus 3 m P	1 m versus 3 m P	Mean (range)	Fresh versus 6 m P	1 m versus 6 m P	3 m versus 6 m P
Th1	10.4 (5.4–19.4)	18.1 (3.4–31.9)	0.0003	11.1 (1.7–26.0)	ns	< 0.0001	13.7 (5.1–22.7)	ns	0.0293	ns
Th2	2.2 (0.7–4.1)	3.5 (1.0–6.1)	0.0048	1.6 (1.0–3.1)	ns	0.0057	1.3 (0.7–2.2)	ns	0.0010	ns
Th17	1.3 (0.5–2.2)	1.5 (0.6–2.3)	ns	1.3 (0.7–1.7)	ns	ns	1.0 (0.7–1.7)	ns	ns	ns
Tfh	10.4 (1.9–20.8)	7.1 (2.2–13.0)	0.0491	9.0 (5.0–15.6)	ns	ns	7.9 (3.0–15.4)	ns	ns	ns
Tregs(CD25⁺CD127^low)	5.1 (2.7–9.2)	2.6 (1.6–3.9)	< 0.0001	2.3 (0.7–4.7)	< 0.0001	ns	2.1 (1.0–3.3)	< 0.0001	< 0.0001	ns
Tregs(CD25⁺Foxp3⁺)	3.8 (2.1–8.2)	1.8 (0.9–2.9)	0.0006	1.1 (0.6–1.8)	< 0.0001	0.0006	1.0 (0.4–2.0)	< 0.0001	0.0006	ns
naïve Tregs/CD4	0.9 (0.3–2.0)	1.0 (0.3–1.6)	ns	0.6 (0.3–1.1)	ns	0.0004	0.4 (0.1–1.0)	0.0026	< 0.0001	0.0003
memory Tregs/CD4	3.1 (1.7–7.0)	0.9 (0.3–1.4)	< 0.0001	0.4 (0.2–0.7)	< 0.0001	< 0.0001	0.7 (0.2–1.8)	< 0.0001	ns	0.0099

ISSN: 1471-2172