Fig. 2
Recombinant human IL-7 stimulation did not affect mCD127 expression on CD8+ T cells, but induced sCD127 release in patients with primary cutaneous melanoma and controls. 104 of peripheral CD8+ T cells (nineteen patients with primary cutaneous melanoma and nine healthy controls) and tissue-infiltrating CD8+ T cells (tumor and para-tumor tissues of seven melanoma patients) were stimulated with recombinant human IL-7 (10 ng/mL) for 48 h in the presence of anti-CD3/CD28. Control cells were cultured with anti-CD3/CD28 only. Cells and supernatants were harvested. a mCD127 expression on CD8+ T cells was investigated by flow cytometry, and the representative flow dots are shown. b There was no significant difference of the percentage of peripheral CD8+ T cells expressing mCD127 between cells with and without IL-7 stimulation in either melanoma patients or controls. c There was no remarkable difference of the percentage of tissue-infiltrating CD8+ T cells expressing mCD127 between cells with and without IL-7 stimulation in either tumor or para-tumor tissues. d IL-7 stimulation enhanced total CD127 mRNA level in peripheral CD8+ T cells in both melanoma patients and controls. e IL-7 stimulation enhanced total CD127 mRNA level in tissue-infiltrating CD8+ T cells in both tumor tissues and para-tumor tissues. f IL-7 stimulation promoted sCD127 mRNA level in peripheral CD8+ T cells in both melanoma patients and controls. g IL-7 stimulation promoted sCD127 mRNA level in tissue-infiltrating CD8+ T cells in both tumor tissues and para-tumor tissues. h IL-7 stimulation promoted sCD127 secretion by peripheral CD8+ T cells in both melanoma patients and controls. i IL-7 stimulation promoted sCD127 secretion by tissue-infiltrating CD8+ T cells in both tumor tissues and para-tumor tissues. Individual level of each subject is shown. Statistical analysis was performed using Student’s t test