Fig. 4

Influence of different concentrations of S100A8/A9, and S100A12 on the chemotaxis and cell death of neutrophils. A The chemotaxis index of ANCA-activated neutrophils treated with different concentrations of S100A8/A9 and S100A12. B Effects of S100A8/A9 and S100A12 on the cell death of ANCA-activated neutrophils. C–K Representative images of the flow cytometry analysis. C Neutrophils were incubated with PBS. D Neutrophils were incubated with normal-IgG. E Neutrophils were incubated with ANCA-IgG alone. F–H Neutrophils were incubated with ANCA combined with 1, 5, and 10 μg/ml S100A8/A9, respectively. I–K Neutrophils were incubated with ANCA combined with 1, 5, and 10 μg/ml S100A12, respectively. *p < 0.05, **p < 0.01, ns: not significant