Fig. 6

S100A8/A9 and S100A12 played their pro-inflammatory effects through the p38 MAPK/NF-κB p65 pathway. The full-length blot of GAPDH in A was absent for the limited exposure space. A S100A8/A9 and S100A12 induced p38 MAPK phosphorylation and NF-κB p65 expression. B The phosphorylation ratios of p38 after neutrophils were stimulated with a combination of ANCA and S100A8/A9 or S100A12. C The activation of NF-κB p65 after neutrophils were stimulated with a combination of ANCA and S100A8/A9 or S100A12. D The phosphorylation of p38 MAPK and the activation of NF-κB p65 after the blockade of TLR4 and RAGE. E The phosphorylation ratio of p38 after blocking TLR4 and RAGE. F The expression of NF-κB p65 after blocking TLR4 and RAGE. *p < 0.05, **p < 0.01, ns: not significant