Fig. 7

Effects of S100A8/A9 and S100A12 on the production of ROS and NETs in AAV. A–I Representative images of influences of S100A8/A9 and S100A12 on ANCA-induced production of ROS. Neutrophils were incubated with different stimulators and analyzed using flow cytometry. A Neutrophils were incubated with PBS. B Neutrophils were incubated with normal-IgG. C Neutrophils were incubated with ANCA. D–F Neutrophils were incubated with ANCA combined with 1, 5, and 10 μg/ml S100A8/A9, respectively. G–I Neutrophils were incubated with ANCA combined with 1, 5, and 10 μg/ml S100A12, respectively. J The mean fluorescence intensity of ROS after neutrophils were incubated with different stimulators. K Effects of S100A8/A9 and S100A12 on ANCA-induced formation of NETs. NE: neutrophil elastase