Fig. 1

Mycobacteria induce activation of the mTOR pathway. A Immunoblots of RAW 264.7 macrophages treated with rapamycin (10 μM) for 3 h or infected with M. smegmatis, BCG, or M. tuberculosis at MOI 10 for 24 h. B Densitometric analysis was calculated for p-S6 or LC3B-II density normalized to β-actin. C Immunoblots of BMDMϕ macrophages infected with M. smegmatis, or BCG at MOI 10 for 24 h. D Densitometric analysis was calculated for p-S6, p-mTOR, or LC3B-II density normalized to β-actin. The mean ± SD of a representative experiment of 3 independent assays is shown. Significance was calculated by one-way ANOVA corrected by Dunnett's Test for multiple comparisons. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001