Fig. 2

Mycobacterial autophagy induction is dose dependent. A Immunoblots of RAW 264.7 macrophages infected with M. smegmatis, BCG, or M. tuberculosis at indicated MOIs 24 h post-infection. B Densitometric phosphorylation analysis for M. smegmatis infection was calculated by p-S6 density ratio to total S6 density. Autophagy densitometric summary analysis for M. smegmatis was calculated by LC3B-II normalized to β-actin. C Densitometric analysis for p-S6 density ratio to total S6 density and LC3B-II normalized to β-actin was calculated for BCG infection (C) and (D) M. tuberculosis. The mean ± SD of a representative experiment of 3 independent assays is shown. Significance was calculated by one-way ANOVA corrected by Dunnett's Test for multiple comparisons. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001