Fig. 4
From: Reversing BCG-mediated autophagy inhibition and mycobacterial survival to improve vaccine efficacy
BCG inhibits autophagy induced by rapamycin and M. smegmatis. A Immunoblots of RAW 264.7 macrophages infected with BCG, M. smegmatis at MOI 10 or left uninfected for 3 h and then treated with indicated concentrations of rapamycin for 16 h. B Densitometric summary analysis was calculated by LC3B-II normalized to β-actin. C Intracellular M. smegmatis survival was determined by enumerating CFU. D BCG survival was monitored by enumerating CFU. The mean ± SD of a representative experiment of 3 independent assays is shown. Significance was calculated by one-way or two-way ANOVA corrected by Dunnett's Test for multiple comparisons. NS, not significant. *p ≤ 0.05, ***p ≤ 0.001, ****p ≤ 0.0001