Fig. 5
From: Reversing BCG-mediated autophagy inhibition and mycobacterial survival to improve vaccine efficacy
Pre-infection of heat-killed and live BCG inhibits M. smegmatis infection-induced autophagy. A Immunoblots of RAW 264.7 macrophages infected with BCG (MOI 10) overnight and then infected with M. smegmatis for 3- or 8-h post-infection. B Densitometric summary analysis was calculated by LC3B-II normalized to β-actin. C Densitometric summary analysis was calculated by p62 normalized to GAPDH. D Densitometric summary analysis was calculated by Beclin normalized to β-actin. E M. smegmatis survival was enumerated by CFU calculation. The mean ± SD of a representative experiment of 3 independent assays is shown. Significance was calculated by one-way corrected by Dunnett's Test or two-Way ANOVA corrected by Bonferroni's Test for multiple comparisons. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001