Fig. 6

BCG lipids are responsible for autophagy inhibition. A Raw 264.7 macrophages were treated with BCG lipids for 16 h and then infected with M. smegmatis for 8 h. B Densitometric summary analysis was calculated by LC3B-II normalized to β-actin and p62 normalized to GAPDH. C M. smegmatis survival was enumerated by CFU. D Raw 264.7 macrophages were treated with M. tuberculosis lipids overnight, and the next day infected with M. smegmatis for 8 h. E M. smegmatis survival after pretreatment with M. tuberculosis total lipids were enumerated by CFU. F RAW-GFP-LC3 cells were infected with M. smegmatis (MOI 10) ± pretreatment with BCG lipids for 16 h. The mean ± SD of a representative experiment of 3 independent assays is shown. Significance was calculated by one-way ANOVA corrected by Dunnett's Test or Student t-test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001