Fig. 5

Cellular and molecular characteristics of immune subtypes. A, C Heatmap for the estimated enrichment scores of 28 immune cell signatures among sarcoma immune subtypes in TCGA (A) and GSE21050 (C) cohorts. Memory B cells, CD56dim natural killer cells and type 17T helper cells are highlighted in red. B, D Differential enrichment scores of memory B cells, CD56dim natural killer cells and type 17T helper cells in TCGA (B) and GSE21050 (D). E Distribution of sarcoma immune subtypes among 6 TCGA pancancer immune subtypes. IMS1 was mainly associated with to the C1 subtype, and the C2 subtype was mainly distributed within IMS2; the percentages of C3 and C4 subtypes in IMS3 were higher than those in IMS1 and IMS2. The percentage of the C6 subtype in IMS3 was lower than those in IMS1 and IMS2. **P < 0.01. F The estimated proportion of 18 significant immune-related features among immune subtypes with FDR < 0.01. The top and bottom of the box are the upper quartile (Q3) and the lower quartile (Q1) of the data, respectively. The solid black line in the box represents the median. The whiskers represent the maximum and minimum values of this group of data. The Kruskal–Wallis test was used to assess significant differences. **P < 0.001, *** P < 0.0001, and **** P < 0.00001