Table 1 Cytokine sequences used in this study
Genes | Sequences | Tm (°C) | |
|---|---|---|---|
IFN- gamma | Forward | TTC TTC AGC AAC AGC AAG GC | 56.2 |
Reverse | ACT CCT TTT CCG CTT CCT GA | 56.2 | |
IL-1α | Forward | CCG TGT TGC TGA AGG AGT TG | 56.4 |
Reverse | GTG CAC CCG ACT TTG TTC TT | 55.9 | |
IL- 6 | Forward | ACA AAG CCA GAG TCC TTC AGA | 56.0 |
Reverse | TGG TCC TTA GCC ACT CCT TC | 56.3 | |
CD 32 | Forward | GAC ACA GCA CCA GTC CAA GA | 57.2 |
Reverse | CAG TTT TGG CAG CTT CTT CC | 54.5 | |
TNF-α | Forward | ACC CTC ACA CTC ACA AAC CA | 56.4 |
Reverse | GGC AGA GAG GAG GTT GAC TT | 56.6 | |
CD 204 | Forward | GCC CCA GAG CAA AAA TAT GA | 53.1 |
Reverse | TGG CTC AAG CTG TTG TCA TC | 55.5 | |
IL-3 | Forward | GCCTGCCTACATCTGCGAAT | 54 |
Reverse | GGTTAGGAGAGACGGAGCCA | 56 | |
IL-10 | Forward | GCTGCCTGCTCTTACTGACT | 54 |
Reverse | GGGGCATCACTTCTACCAGG | 56 | |
TGF-β | Forward | GCTGAGCACCTTTTTGCTCC | 54 |
Reverse | GATGGCATTTTCGGAGGGGA | 54 | |
iNOS | Forward | CTATGGCCGCTTTGATGTGC | 59.97 |
Reverse | CAACCTTGGTGTTGAAGGCG | 59.97 | |
GAPDH | Forward | CGT CCC GTA GAC AAA ATG GT | 54.9 |
Reverse | TTG ATG GCA ACA ATC TCC AC | 53.3 | |