Fig. 2

Bregs were decreased in sepsis patients on day 1. A Gating strategy of Bregs in representative healthy and sepsis patients. > 1 million events in the lymphocyte gate were collected. B Bregs requency in total B cells and C absolute number per microlitre of blood of Bregs in all healthy and sepsis subjects. Absolute number of Bregs per microlitre of blood was calculated by multiplying the total number of B lymphocytes calculated by the percentage of positive cells in Bregs in lymphocytes. D Frequency and E absolute number of Bregs in sepsis patients caused by digestive tract, lung, biliary tract, urinary tract, and skin/soft tissue infection. F Frequency and G absolute number of Bregs in sepsis patients with bacterial, viral, and fungal causes. Two-tailed t-test with unequal variance or Kruskal–Wallis test. ****p < 0.0001