Fig. 1

Flow cytometry analysis of CD4⁺ T cell subsets in the tumor draining lymph nodes of patients with HNSCC. Lymphocytes were gated A CD4⁺ T cells were gated followed by defining these subpopulations in CD4⁺ T cells gate: B TNF-α⁺ cells, C (1)TNF-αhi cells, (2) TNF-αlow cells, D IFN-γ⁺ cells, E (1) IFN-γ⁺TNF-α⁺ cells, (2) IFN-γ⁺TNF-α⁻ cells, F IL-4⁺ cells, G IL-17⁺ cells, H IL-10⁺ cells, I TGF-β⁺ cells subsets according to their cognate cytokine expression and J (1)CD25⁺Foxp3⁺ cells, (2) CD25⁻Foxp3⁺ cells, K CD127 ^low/− cells subsets according to CD25, Foxp3 and CD127 expression and L (1) CD25⁺Foxp3⁺ cells, (2) CD25⁻Foxp3⁺ cells in CD127^low/− cells gate