Fig. 2

Flow cytometry analysis of CD8⁺ T cell subsets in the tumor draining lymph nodes of patients with HNSCC. A Lymphocytes were gated, B CD8⁺ T cells were gated followed by defining these subpopulations in CD8⁺ T cells gate: C TNF-α⁺ cells, D IFN-γ⁺ cells, E (1) IFN-γ⁺TNF-α⁺ cells, (2) IFN-γ⁺TNF-α⁻ cells, F IL-4⁺ cells, G IL-17⁺ cells, H IL-10⁺ cells, I TGF-β⁺ cells subsets according to their cognate cytokine expression