Fig. 1

The protein expressions of IL-1β, IL-6, TNF-α, IL-10, PU.1, CD23, p-ERK, CCL20 and IL-8 in human THP-1-derived macrophages with AF infection within 24 h. A-D ELISA assay showed the levels of the inflammatory factors IL-1β (A), IL-6 (B), TNF-α (C) and IL-12 (D) in HTMs with AF infection. E Western blotting analysis showed the protein expressions of PU.1, CD23, p-ERK, CCL20 and IL-8 in HTMs with AF infection normalized to GAPDH. The samples derived from the same experiment and that gels/blots were processed in parallel. F–J Quantitative analysis of the expressions of PU.1, CD23, p-ERK, CCL20 and IL-8 in E. The X axis represents the infection time. All data are presented as the mean ± SD, N ≥ 3, *P < 0.05, **P < 0.01