Fig. 1

The effects of different miR-15a-5p inhibitors on the expression of miR-15a-5p in primary ECs. ECs were transfected with Lipofectamine RNAiMAX in complex with A mirVana, B LNA-PE or C LNA-PS miR-15a-5p inhibitors and their respective scrambled control at elevated concentration (9, 30 and 100 nM). Following 48 h, total RNAs were isolated and expression levels of miR-15a-5p and reference miRNA miR-103a-3p were measured by means of qRT-PCR. D MiR-15a-5p expression following two consecutive transfections, 24 h each, with 30 nM oligonucleotide-lipid complexes. E MiR-15a-5p expression in primary ECs following 24 h transfection using oligonucleotide-lipid complexes at 30 nM. Relative miR-15a-5p expression was shown as mean ± SEM. N = 3 experiments and N = 2 technical replicates were performed